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. 2018 Jan 6;102(4):562–569. doi: 10.1136/bjophthalmol-2017-311150

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© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

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Mechanical stimulation and inducing medium generate a transparent and multilamellar cell sheet. PDLSCs were seeded on collagen I bonded Bioflex 6-well plates and exposed to static mechanical strain and inducing medium for 12 days to form a cell sheet. The collagen membrane of the Bioflex 6-well plate itself (A) and the collagen membrane with the formed cell sheet (B) were both transparent. (C) The light transmission of the cell sheet was measured in the wavelength from 400 nm to 850 nm. (D) The formed cell sheet was found to be a multilamellar structure, as revealed by DAPI staining; white arrows indicating nuclei that are focused on one layer, and red arrows indicating nuclei that were not focused and thus on another layer. PDLSCs, periodontal ligament stem cells.