Table 1.
Primers and PCR conditions used to verify gene mutations in d-cycloserine-resistant mutants
| Gene | Primer | Sequence (5′–3′) |
|---|---|---|
| alr | alr_F2 | GAAAATAAAAGACACGCCTACTTTCGCTCCA |
| alr_R2 + 300bp | GACATCCATCGCCATGGCAATACCCTT | |
| rv0759c | Rv0759c-For | AAAGCCGAAATCACTGAGGCTGCGGG |
| Rv0759c-Rev | TAAGGAGGCCGTCGGCGCCTTCTTC | |
| rv1403c | Rv1403c-For | AATTCGCCGGCGCTAAACGGGAGG |
| Rv1403c-Rev | ATCAGTTCGGCGCCGACCAACCG | |
| rv1435c | Rv1435c-For | AGCCGCCGCGTCCGGGCTTAA |
| Rv1435c-Rev | AGGCCCGGTAGAAGTTGCGTCCGAT | |
| rv1726 | Rv1726-F | AGTACTGGCCGTACGGCTGCA |
| Rv1726-R | GAGGAGTAGCTGTCGGCTTCTATCG | |
| rv1731 | Rv1731-For | GGCCGGTTCTTCGTGGTAACGTGCC |
| Rv1731-Rev | CTGTGCCTTACGGGGCTTCAGCAGG | |
| rv2831 | Rv2831-F | GCCACTTCATCAAGCAAGGAGAG |
| Rv2831-R | CTCTTCAACAGCCGCACCGAG | |
| rv3331 | Rv3331-For | ACACTGATCGAACCCGACCCGTCGC |
| Rv3331-Rev | CCGTGGTGGTCAGCAACTCCTGTTCTC | |
| rv3690 | Rv3690-F | GTATTGCAGACCGGCTTAGAAGCC |
| Rv3690-R | GTCAAATAGGTGCCGATCGAGG | |
| rv3772 | Rv3772-For | TGAGCTACGGGCGCTTGTCTTCAGTTG |
| Rv3772-Rev | GTGTGGTCGGTCAACGGCACCTGGA |
Conditions: 95 °C for 5 min; 35 turns of 94 °C for 30 s, 62 °C for 30 s and 72 °C for 90 s; and finally 72 °C for 5 min.