Table 2.
Mutations identified in d-cycloserine-resistant mutants of M. tuberculosis by WGS analysis
| Strain ID | MIC of cycloserine (mg/L) | Mutation type | Locus_tag | Nucleotide mutation | Amino acid change | Gene product |
|---|---|---|---|---|---|---|
| DT69-1 | 40 | non-synonymous | alr (rv3423c) | C1030T | D344N | Alr |
| DT61-1 | 40 | intergenic SNV | PE_PGRS50 (rv3345c) | G(−52)T | — | PE family |
| non-synonymous | alr (rv3423c) | C1030T | D344N | Alr | ||
| DT16-2 | 20 | non-synonymous | rv0059 | C278T | A93V | hypothetical protein (heterogeneous) |
| DT63-1 | 20 | non-synonymous | rv0221 | C166T | P56S | acyltransferase, wax ester synthase, diacylglycerol acyltransferase and monoacylglycerol acyltransferase (WS/DGAT/MGAT) (heterogeneous) |
| DT72-1 | 20 | intergenic insertion | rv0759c | (−27) Ins C | — | hypothetical protein |
| DT71-1 | 20 | non-synonymous | betP (rv0917) | T941G | L314R | betaine–carnitine–choline transporter (BCCT) family transporter |
| DT1-1 | 20 | non-synonymous | rv1403c | T19C | T7A | methyltransferase |
| DT76-1 | 20 | intergenic SNV | rv1435c | T(−150)C | — | hypothetical protein |
| DT82-4 | 20 | intergenic SNV | rv1435c | T(−150)C | — | hypothetical protein |
| DT15-2 | 20 | non-synonymous | rv1683 | G1126A | A376T | long-chain acyl-CoA synthase |
| DT81-2-1 | 20 | non-synonymous | rv1726 | T440C | V147A | oxidoreductase |
| DT81-2 | 20 | non-synonymous | rv1726 | T440C | V147A | oxidoreductase |
| DT79-6 | 20 | non-synonymous | gabD2 (rv1731) | A92C | E31A | succinate-semialdehyde dehydrogenase |
| DT9-1 | 20 | non-synonymous | rv2749 | C4T | P2S | hypothetical protein (heterogeneous) |
| DT21-5 | 20 | non-synonymous | sugI (rv3331) | C16T | Q6stop | sugar-transport integral membrane protein, SugI, MFS transporter |
| DT6-6 | 20 | intergenic SNV | PE_PGRS50 (rv3345c) | G(−52)T | — | PE family |
| DT3-2 | 20 | non-synonymous | rv3690 | G103A | D35N | hypothetical protein |
| non-synonymous | echA16 (rv2831) | G253T | A85S | enoyl-CoA hydratase | ||
| DT12-2 | 20 | non-synonymous | hisC2 (rv3772) | T110C | L37P | histidinol-phosphate aminotransferase (heterogeneous) |