Figure 2.

T. gondii ME49 forms tissue cysts after oral oocyst inoculation. Brain pieces of dams inoculated with five oocysts and euthanized after 6 weeks post-infection (see Table 1 and Figure 1) were processed for immunofluorescence performed with anti-T. gondii-BAG1 (BAG, green) and anti-T. gondii antiserum (Toxo, red) as described in the Materials and methods section. The blue dye DAPI indicates host cell and parasite nuclei. Arrows in the overlay point to the cyst wall stained with anti-T. gondii antiserum, which is not labelled by BAG1 antibody. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC.