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In vitro growth in competition assay for the three mutant strains (a) CRG20291-TA (open squares), (b) CRG20291-TG (open triangles) and (c) CRG20291-GT (filled triangles) compared with the parental strain of C. difficile (filled circles). Each mutant was grown in competition with the parental strain and fitness was evaluated over 24 h. Values are plotted as mean ± SD.
