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Northern-blot analyses of IAA1 and IAA2 steady-state mRNA levels in wild-type seedlings (wt, s), wild-type calli (wt, c), and mutant calli. Total RNA (50 μg) was separated in a denaturing formaldehyde gel and, after blot transfer to a nylon filter, hybridized with 32P-labeled specific cDNA probes. Hybridization with a 25S rDNA probe served as a control for loading.
