Table II.
Content of free and conjugated IAA in wild-type (WT) seedlings and mutant calli
| Plant Material | IAA | IAA Conjugates |
|---|---|---|
| pmol g−1 fresh wt | ||
| WT seedling | 12.7 ± 4.6 | 13,400 ± 5,300 |
| Callus | ||
| r1 | 295 ± 41 | 430,000 ± 173,000 |
| r2 | 205 ± 44 | 179,000 ± 23,000 |
| r6 | 121 ± 12 | 71,000 ± 19,000 |
The mutant calli and WT seedlings were grown in vitro on solidified, hormone-free MS medium. IAA and IAA conjugate concentrations were determined by gas chromatography-selected ion reactant-MS as described in “Materials and Methods.” The data are the means ± se of three independent biological replications.