Table III.
Cytokinin content of mutant line c1 compared to wild-type (WT) seedlings
| Cytokinin Metabolite | WT Seedling | c1 Callus | Increaseb |
|---|---|---|---|
| pmol g−1 fresh wt | |||
| iP | 7.6a | 1.2 ± 0.1 | 0.2 |
| iP Riboside | 3.7 ± 3.3 | 22.7 ± 5.4 | 6 |
| iP Riboside 5′-monophosphate | 1.5 ± 0.1 | 12.5 ± 0.4 | 8 |
| iP 9-Glucoside | 4.7 ± 2.2 | 25.7 ± 4.3 | 6 |
| trans-Zeatin | 0.8a | 0.65 ± 0.0 | 1 |
| Zeatin riboside | 2.2 ± 1.7 | 42.9 ± 5.4 | 20 |
| Zeatin riboside 5′-monophosphate | 2.4 ± 1.3 | 11.4 ± 2.0 | 5 |
| Zeatin O-glucoside | 6.5 ± 1.0 | 525 ± 105 | 81 |
| Zeatin riboside O-glucoside | 2.3 ± 0.3 | 14.8 ± 0.0 | 6 |
| Zeatin 7-glucoside | 148 ± 26 | 751 ± 25 | 5 |
| Zeatin 9-glucoside | 21 ± 20 | 257 ± 50 | 12 |
| Dihydrozeatin | 1.4a | 0.2 ± 0.1 | 0.2 |
| Dihydrozeatin riboside | 1.1 ± 0.7 | 2.8 ± 0.3 | 3 |
| Dihydrozeatin riboside 5′-monophosphate | 1.0 ± 0.3 | 1.4 ± 0.0 | 1 |
| Dihydrozeatin O-glucoside | 7.1 ± 2.5 | 322 ± 46 | 45 |
| Dihydrozeatin riboside O-glucoside | 2.3 ± 0.3 | 11.7 ± 3.1 | 5 |
| Dihydrozeatin 7-glucoside | 2.8 ± 1.6 | 15.1 ± 7.2 | 5 |
| Dihydrozeatin 9-glucoside | 9.7 ± 9.1 | 13.9 ± 0.0 | 1 |
Mutant calli and wild-type seedlings (wt, s) were grown in vitro on solidified hormone-free MS medium. Cytokinin concentrations were determined by micro liquid chromatography with column switch configuration coupled to positive ion electrospray tandem mass spectrometry using multiple reactant monitoring as described in “Materials and Methods.” The data are the means ± se of two biological independent replications.
a
Data from a single sample.
b
Increase in c1 callus compared with wild-type seedlings.