Table IV.
Cytokinin oxidase activity in mutant lines
| Plant Material | Cytokinin Oxidase Activity |
|---|---|
| pmol mg−1 protein h−1 | |
| Seedling (WT) | 11.4 ± 1.7 |
| Callus | |
| WT | 4.9a |
| c1 | 0.6 ± 0.3 |
| c2 | 4.5 ± 2.5 |
| s1 | 4.5 ± 0.5 |
| r1 | 4.8 ± 0.7 |
Cytokinin oxidase assays were based on the rate of conversion of [2,8-3H]iP to adenine. Substrate and product were separated by thin-layer chromatography. Radioactivity was measured using a liquid scintillation counter. Other details are described in “Materials and Methods.” Wild-type (WT) seedlings and mutant calli were grown on solidified, hormone-free MS medium. WT callus was grown on 1 mg/L naphthylacetic acid and 0.1 mg/L iP. The values represent the means ± se of three independent samples.
a
Data from a single replicate.