Table 3.
Time to regrowth, antibiotic concentration and live biomass of 24 and 72 h PAO1 biofilms during treatment with meropenem and tobramycin based on the parameter estimates reported in Table 2
| 24 h PAO1 |
72 h PAO1 |
|||||||
|---|---|---|---|---|---|---|---|---|
| time to regrowth |
antibiotic concentration (mg/L) | biomass (μg3/μm2) | time to regrowth |
antibiotic concentration (mg/L) | biomass (μg3/μm2) | |||
| h | % dosing interval | h | % dosing interval | |||||
| Meropenem | ||||||||
| dose 1 | 3.5 | 43.8 | 7.30 | 0.15 | 3.5 | 43.8 | 7.30 | 20.8 |
| dose 2 | – | – | – | 3.0 | 37.5 | 10.7 | 16.9 | |
| dose 3 | – | – | – | 2.5 | 31.25 | 15.8 | 14.6 | |
| Tobramycin | ||||||||
| dose 1 | 7.5 | 31.25 | 4.9 | 0.057 | 6.5 | 27.0 | 5.61 | 20.3 |
| dose 2 | – | – | – | 6.5 | 27.0 | 6.39 | 19.3 | |
| dose 3 | – | – | – | 6 | 25.0 | 7.2 | 19.0 | |
| Meropenem plus tobramycin | – | – | – | 12 | 50 | meropenem 17.0; tobramycin 1.59 | 7.99 | |
Time to regrowth represents the duration of time for biofilm cells to restart growing during antibiotic treatment. The concentration at regrowth represents the meropenem or tobramycin concentration in the flow chambers when regrowth occurs.