Table 1.

Characteristics of the E. coli and K. georgiana strains used in this study

Strains	Characteristics	MIC with G6P (mg/L)	MIC without G6P (mg/L)	Zone diameter (mm)	Zone diameter with PPF (mm)
E. coli YD472	contains fosA3 on plasmid	>1024	>1024	6	6
K. georgiana YDC799	contains fosAKG on the chromosome	32	>1024	18	24
K. georgiana ATCC 51603	contains fosAKG on the chromosome	0.5	128	32	34
E. coli TOP10 (pFosA3)	carries fosA3 from YD472 on pBC-SK	>1024	>1024	6	18
E. coli TOP10 (pFosAKG-YDC799)	carries fosAKG from YDC799 on pBC-SK	>1024	>1024	6	18
E. coli TOP10 (pFosAKG-ATCC 51603)	carries fosAKG from ATCC 51603 on pBC-SK	>1024	>1024	6	22
E. coli TOP10 (pBC-SK)	vector control	1	128	40	40
E. coli TOP10		1	64	38	38

MICs were determined by the agar dilution method with and without 25 mg/L glucose-6-phosphate (G6P). For disc testing, 1 mg of sodium phosphonoformate (PPF) was added to fosfomycin discs.