Figure 2.
Effect of synchrony on drug susceptibility measured across the life cycle using the SYBR Green I method after one or two life cycles. EC50 values for chloroquine (squares), dihydroartemisinin (circles) or pyrimethamine (diamonds) were determined from experiments initiated at the times shown on the y-axis using synchronized parasites at 1% parasitemia and 1% haematocrit that were incubated for either 27 h (a) or 54 h (b) for P. knowlesi, and 48 h (c) or 96 h (d) for P. falciparum. Signal window (squares) and assay quality (Z′ factor, circles) (e–h) were determined as in Figure 1.