Table 3.
Delayed death effect of three antibacterial agents against P. knowlesi and P. falciparum assessed using the SYBR Green I assay
| Antibacterial |
P. knowlesi EC50 (nM) |
P. falciparum EC50 (nM) |
||||
|---|---|---|---|---|---|---|
| 27 h | 81 h | fold difference | 48 h | 96 h | fold difference | |
| Azithromycin | 5662 ± 725 | 31.9 ± 10 | 177 | 6003 ± 323 | 19.2 ± 4 | 313 |
| Doxycycline | >10000 | 2061 ± 343 | >4.9 | >10000 | 623 ± 148 | >16 |
| Clindamycin | >10000 | 15.9 ± 4 | >629 | >10000 | 7.0 ± 1.0 | >1429 |
All antibacterial agents were screened in duplicate on at least three separate occasions from a starting parasitaemia and haematocrit of 1%. The EC50 values are reported as the mean ± SEM. The fold difference for each compound is calculated by dividing the EC50 value after three life cycles (for P. knowlesi) or two life cycles (for P. falciparum) by the EC50 value measured after a single life cycle exposure.