Table 4.
Comparison of the susceptibility of P. knowlesi grown in either human or macaque red blood cells to selected antimalarial agents assessed using the SYBR Green I assay
| Antimalarial | EC50 (nM) |
Fold difference | P | |
|---|---|---|---|---|
| human blood | macaque blood | |||
| Chloroquine | 24.6 ± 3.3 | 42.0 ± 8.1 | 0.59 | 0.2114 |
| Dihydroartemisinin | 1.9 ± 0.2 | 4.1 ± 0.7 | 0.46 | 0.1250 |
| Quinine | 48.0 ± 6.7 | 54.7 ± 1.4 | 0.88 | 0.4303 |
| Mefloquine | 11.0 ± 1.5 | 18.3 ± 0.2 | 0.60 | 0.0502 |
| Pyrimethamine | 8.9 ± 0.8 | 13.2 ± 2.7 | 0.67 | 0.2940 |
Antimalarial agents were screened in duplicate on three separate occasions from a starting parasitaemia and haematocrit of 1%. The EC50 values are reported as the mean ± SEM. The fold difference for each compound is calculated by dividing the EC50 in human blood by the EC50 value in macaque blood measured after a single life cycle exposure (27 h).