Table 1.
Primer sequences
| Primer | Sequence 5′ to 3′ | Target/purpose |
|---|---|---|
| traG_for | GAATAACCTTGGTTTGGCTTCGG | traG |
| traG_rev | CATACACGAGCATGAGCGAAATTG | |
| nadV_for | CTGTATGAGATTTAAGGAAAGAAATTATTATGGATAACC | nadV |
| nadV_rev | GCGTATTAAGTACAAATATCATAGCGTAGTGC | |
| floR_for | CGACGCCCGCTATGATCCAACTC | floR RT–PCR |
| floR_rev | CCCAAAAAGCCGGACTCGCGAAG | |
| IceFlo_L1_out | GCGAACGTCTTACCATCATTTCTC | circular forms and insertion sites of ICEApl2 |
| IceFlo_R1_out | GATTGGACTGAAATAGCGGAGG | |
| IceFlo_L2_out | GTGGTTTTAAGCGTTGAAAGGC | |
| IceFlo_R2_out | GGTAGCGAAGGAATTTGTGGTTTAG | |
| yhaH_5′_out | GCGTAAAGCATAGATAAACCACTCC | |
| prfC_5′_out | GCGAGATGATAGCAAAGGTCC | |
| FloR_start_fora | GAGGGTTGATTCGTCATGACCA | cloning of floR genes |
| FloR_down_rev | GGATACCGACATTCACGAGGTTTC | |
| FloR_TAmut_for | CCGGTATGGGCACCTACTTCGTCTTCTTCTCG | site-directed mutagenesis |
| FloR_TAmut_rev | CGAGAAGAAGACGAAGTAGGTGCCCATACCGG | |
| FloR_CTmut_for | CCCTATCGCCGGAGTATTGATCGGCGAGTTC | |
| FloR_CTmut_rev | GAACTCGCCGATCAATACTCCGGCGATAGGG |
a
In the sequence of primer FloR_start_for, the bold letters indicate the RBS and the italics indicate the start codon for the floR gene.