Fig. 3. trans-Cleavage activity of Dz7-38-32t. (A) Hypothetical 2D structures of Dz7-38-32t (bottom sequence: 3′–5′) in complex with the 19 nt HIV-1 LTR-promoter mRNA (top sequence in red: 5′–3′). The cleavage site is indicated by the arrow. All the three modified nucleosides (A, C, and U) are in blue. (B and C) Multiple-turnover activities at 30 °C (left panel) and 37 °C (right panel), respectively. (B) Representative autoradiographic images of trans-cleavage activity ([D7-38-32t] = 5 nM, [substrate] = 500 nM); (C) Multiple-turnover profiles generated by fitting obtained kobs values and substrate concentrations in Michaelis–Menten equation. Data are the mean of three replicative experiments, with R2 ≥ 0.98 in both cases.
