Table I.
Ab clones and fluorophores
| Ab | Clone | Fluorophore | Source |
|---|---|---|---|
| CD16/32 | 93 | eBioscience | |
| CD45 | 30-F11 | Alexa Fluor 488 | BioLegend |
| TCRβ | H57-597 | PerCP/Cy5.5 | BioLegend |
| CD4 | RM4-5 | BV 510 | BioLegend |
| CD8 | 53-6.7 | APC-eFluor 780 | eBioscience |
| CD44 | IM7 | Alexa Fluor 700 | BioLegend |
| CD25 | PC61 | PE Dazzle | BioLegend |
| CD64 | X54-5/7.1 | Alexa Fluor 647 | BioLegend |
| CD11b | M1/70 | BV 421 | BioLegend |
| CD11c | N418 | BV 570 | BioLegend |
| Ly6C | HK1.4 | PE-Cy7 | BioLegend |
| Ly6G | 1A8 | BV 711 | BioLegend |
| NK1.1 | PK136 | PE | BioLegend |
| MHC II | M5/114.15.2 | BV 785 | BioLegend |
| PD-1 | 29F.1A12 | BV 605 | BioLegend |
| CD86 | GL-1 | BV 650 | BioLegend |
Abs were diluted 1:100 and incubated with cells from tumors, dLN, and spleen.