Fig. 1.

Alkyne tagged fatty acids. Average spectra of three alkyne tagged fatty acids and their structures (a) and relative intensity (mean + standard deviation) of the alkyne stretching frequency at 2110 cm⁻¹ to the total spectral intensity between 1181 cm⁻¹ and 2740 cm⁻¹ for each tagged fatty acid (b). Black = C14 tag; pink = C16 tag; blue = C18 tag, dashed line in (a) indicates peak position of 2110 cm⁻¹. Spectra were acquired using 532 nm excitation, 50%/ca. 10 mW laser power, 10 s acquisition time and an extended spectral range of 200 cm⁻¹ to 3200 cm⁻¹. Three spectra were acquired and min-max scaled for each compound and the average spectrum was plotted for each compound. Spectra were offset for clarity.