. 2017 Feb 3;41(2):83–99. doi: 10.1093/jat/bkx012

Table I.

Analyses of blood and oral fluid specimens following oral consumption of cannabis brownies

Subject #	Time (h)^a	THC dose (mg)	Blood THCCOOH ELISA (cutoff = 10 ng/mL)^b	Blood THC LC–MS-MS (ng/mL)	Blood 11-OH-THC LC–MS-MS (ng/mL)	Blood THCCOOH LC–MS-MS(ng/mL)	Oral fluid THC ELISA (cutoff = 4 ng/mL)	Oral fluid THCCOOH ELISA (cutoff = 0.050 ng/mL)	Oral fluid THC LC–MS-MS (ng/mL)	Oral fluid THCCOOH LC–MS-MS (ng/mL)
1	−1	10	NEG	0	0	0	NEG	NEG	0	0
1	0.17	10	NEG	0	0	0	POS	POS	144	0
1	0.5	10	NEG	0	0	0	POS	POS	18	0
1	1	10	NEG	0	0	0	POS	NEG	2	0
1	1.5	10	POS	0	0	0	POS	NEG	2	0
1	2	10	POS	0	0	4	NEG	NEG	0	0
1	3	10	POS	0	0	5	NEG	NEG	0	0
1	4	10	POS	0	0	0	NEG	NEG	0	0
1	5	10	POS	0	0	0	NEG	NEG	0	0
1	6	10	POS	0	0	0	NEG	NEG	0	0
1	8	10	POS	0	0	0	NEG	NEG	0	0
1	12	10	POS	0	0	0	NEG	NEG	0	0
1	22	10	POS	0	0	0	NEG	NEG	0	0
1	26	10	POS	0	0	0	NEG	NEG	0	0
1	30	10	POS	0	0	0	NEG	NEG	0	0
1	34	10	POS	0	0	0	NEG	NEG	0	0
6	−1	10	NEG	0	0	0	NEG	NEG	0	0
6	0.17	10	NEG	0	0	0	POS	POS	263	0
6	0.5	10	NEG	0	0	0	POS	NEG	80	0
6	1	10	POS	1	1	6	POS	POS	28	0
6	1.5	10	POS	1	1	8	POS	NEG	14	0
6	2	10	POS	1	0	6	POS	NEG	5	0
6	3	10	POS	1	1	10	NEG	NEG	2	0.231
6	4	10	POS	1	1	14	NEG	NEG	0	0
6	5	10	POS	1	0	8	NEG	NEG	0	0.045
6	6	10	POS	0	0	5	NEG	NEG	0	0.029
6	8	10	POS	0	0	0	NEG	NEG	0	0.037
6	12	10	POS	0	0	0	NEG	NEG	0	0
6	22	10	POS	0	0	0	NEG	NEG	0	0
6	26	10	POS	0	0	0	NEG	NEG	0	0
6	30	10	POS	0	0	0	NEG	NEG	0	0
12	−1	10	NEG	0	0	0	NEG	NEG	0	0
12	0.17	10	NEG	0	0	0	POS	POS	47	0
12	0.5	10	NEG	0	0	0	POS	NEG	3	0
12	1	10	NEG	1	1	0	POS	NEG	2	0
12	1.5	10	POS	2	1	0	POS	NEG	4	0
12	2	10	POS	3	2	0	NEG	NEG	0	0
12	3	10	POS	3	2	5	NEG	NEG	0	0
12	4	10	POS	0.8	1	0	NEG	NEG	0	0
12	5	10	POS	0	0	0	NEG	NEG	0	0
12	6	10	POS	0	0	0	NEG	NEG	0	0
12	8	10	POS	0	0	0	NEG	NEG	0	0
12	12	10	POS	0	0	0	NEG	NEG	0	0
12	22	10	POS	0	0	0	NEG	NEG	0	0
12	26	10	POS	0	0	0	NEG	NEG	0	0
12	30	10	POS	0	0	0	NEG	NEG	0	0
12	34	10	POS	0	0	0	NEG	NEG	0	0
15	−1	10	NEG	0	0	0	NEG	NEG	0	0
15	0.17	10	NEG	0	0	0	POS	POS	412	0.021
15	0.5	10	NEG	0	0	0	POS	NEG	139	0
15	1	10	NEG	0	1	3	POS	NEG	52	0
15	1.5	10	NEG	1	1	4	POS	NEG	8	0
15	2	10	POS	1	1	5	NEG	NEG	2	0.025
15	3	10	POS	0	1	5	NEG	NEG	0	0.074
15	4	10	POS	0	0	4	NEG	NEG	0	0.02
15	5	10	POS	0	0	0	NEG	NEG	0	0.024
15	6	10	POS	0	0	0	NEG	NEG	0	0.026
15	8	10	NEG	0	0	0	NEG	NEG	0	0.044
15	12	10	NEG	0	0	0	NEG	NEG	0	0
15	22	10	POS	0	0	0	NEG	NEG	0	0
20	−1	10	NEG	0	0	0	NEG	NEG	0	0
20	0.17	10	NEG	0	0	0	POS	POS	161	0
20	0.5	10	NEG	0	0	0	POS	POS	199	0
20	1	10	POS	0.8	1	5	POS	POS	48	0
20	1.5	10	POS	0	1	6	POS	NEG	15	0
20	2	10	POS	0	0	6	POS	POS	15	0
20	3	10	POS	0	0	8	NEG	NEG	0	0
20	4	10	POS	0	0	5	NEG	NEG	0	0
20	5	10	POS	0	0	0	NEG	NEG	0	0
20	6	10	POS	0	0	5	NEG	NEG	0	0
20	8	10	POS	0	0	4	NEG	NEG	0	0
20	12	10	POS	0	0	3	NEG	NEG	0	0
20	22	10	POS	0	0	0	NEG	NEG	0	0
20	26	10	POS	0	0	0	NEG	NEG	0	0
20	30	10	POS	0	0	0	NEG	NEG	0	0
20	34	10	POS	0	0	0	NEG	NEG	0	0
20	46	10	POS	0	0	0	NEG	NEG	0	0
20	50	10	POS	0	0	0	NEG	NEG	0	0
20	54	10	POS	0	0	0	NEG	NEG	0	0
22	−1	10	NEG	0	0	0	NEG	NEG	0	0
22	0.17	10	NEG	0	0	0	POS	POS	84	0
22	0.5	10	NEG	0	0	0	POS	NEG	8	0
22	1	10	NEG	0	0	0	NEG	NEG	0	0
22	1.5	10	NEG	0	0	0	NEG	NEG	2	0
22	2	10	POS	0	0	3	NEG	NEG	0	0
22	3	10	POS	0	1	5	NEG	NEG	0	0
22	4	10	POS	0	0	6	NEG	NEG	0	0
22	5	10	POS	0	0	4	NEG	NEG	0	0
22	6	10	POS	0	0	3	NEG	NEG	0	0
22	8	10	POS	0	0	3	NEG	NEG	0	0
22	12	10	NEG	0	0	2	NEG	NEG	0	0
5	−1	25	NEG	0	0	0	NEG	NEG	0	0
5	0.17	25	NEG	0	0	0	POS	POS	1128	0.098
5	0.5	25	NEG	0	0	0	POS	POS	310	0
5	1	25	POS	1	0	7	POS	NEG	49	0
5	1.5	25	POS	1	1	12	POS	NEG	30	0
5	2	25	POS	2	1	15	POS	POS	19	0.135
5	3	25	POS	1	1	20	POS	POS	3	0.251
5	4	25	POS	3	2	20	NEG	POS	0	0
5	5	25	POS	1	0	14	NEG	POS	0	0.217
5	6	25	POS	1	0	16	NEG	POS	0	0.153
5	8	25	POS	0	0	5	NEG	NEG	0	0.081
5	12	25	POS	0	0	0	NEG	NEG	0	0
5	22	25	POS	0	0	0	NEG	POS	0	0
5	26	25	POS	0	0	0	NEG	NEG	0	0
5	30	25	POS	0	0	0	NEG	NEG	0	0.027
5	34	25	POS	0	0	0	NEG	NEG	0	0
5	46	25	POS	0	0	0	NEG	NEG	0	0
5	50	25	POS	0	0	0	NEG	POS	0	0.096
5	54	25	NEG	0	0	0	NEG	NEG	0	0
5	58	25	NEG	0	0	0	NEG	NEG	0	0
5	70	25	NEG	0	0	0	NEG	NEG	0	0.053
5	74	25	NEG	0	0	0	NEG	NEG	0	0.026
8	−1	25	NEG	0	0	0	NEG	NEG	0	0
8	0.17	25	NEG	0	0	0	NS	NS	NS	NS
8	0.5	25	NEG	2	2	7	POS	POS	213	0
8	1	25	POS	2	3	21	NS	NS	NS	NS
8	1.5	25	MS	NS	NS	NS	NS	NS	NS	NS
8	2	25	POS	1	2	27	POS	NEG	23	0.115
8	3	25	POS	4	5	39	POS	NEG	4	0.092
8	4	25	POS	1	2	22	POS	NEG	0	0.091
8	5	25	POS	2	3	28	NEG	NEG	0	0.079
8	6	25	POS	0	4	39	NEG	POS	2	0.209
8	8	25	POS	0	2	25	NEG	NEG	0	0.061
8	12	25	POS	0	0	11	NEG	NEG	0	0.038
8	22	25	POS	0	0	12	NEG	NEG	0	0.204
8	26	25	POS	0	0	0	NEG	NEG	0	0
8	30	25	POS	0	0	8	NEG	NEG	0	0
8	34	25	POS	0	0	8	NEG	NEG	0	0
8	46	25	POS	0	0	9	NEG	NEG	0	0
8	50	25	POS	0	0	0	NEG	NEG	0	0
8	54	25	POS	0	0	0	NEG	NEG	0	0
8	58	25	POS	0	0	0	NEG	NEG	0	0
9	−1	25	NEG	0	0	0	NEG	NEG	0	0
9	0.17	25	NEG	0	0	0	POS	POS	70	0
9	0.5	25	POS	1	1	3	POS	POS	51	0
9	1	25	POS	2	1	9	POS	NEG	12	0
9	1.5	25	POS	2	1	9	POS	NEG	2	0
9	2	25	POS	3	1	14	POS	NEG	2	0
9	3	25	POS	2	1	15	NEG	NEG	0	0
9	4	25	POS	2	2	16	NEG	NEG	0	0.032
9	5	25	POS	0	1	11	NEG	NEG	0	0
9	6	25	POS	0	0	10	NEG	NEG	0	0
9	8	25	POS	0	0	8	NEG	POS	0	0.101
9	12	25	POS	0	0	8	NEG	NEG	0	0
9	22	25	POS	0	0	5	NEG	NEG	0	0
9	26	25	POS	0	0	6	NEG	NEG	0	0
9	30	25	POS	0	0	2	NEG	NEG	0	0
9	34	25	POS	0	0	0	NEG	NEG	0	0
9	46	25	POS	0	0	0	NEG	NEG	0	0
9	50	25	POS	0	0	0	NEG	NEG	0	0
11	−1	25	NEG	0	0	0	NEG	NEG	0	0
11	0.17	25	NEG	0	0	0	POS	POS	400	0
11	0.5	25	POS	0	2	0	POS	POS	125	0
11	1	25	POS	4	4	17	NS	NS	47	0
11	1.5	25	POS	2	5	23	POS	NEG	4	0.021
11	2	25	POS	1	2	19	POS	NEG	6	0.037
11	3	25	POS	1	2	13	NEG	NEG	0	0.036
11	4	25	POS	0	1	12	NEG	NEG	0	0.055
11	5	25	POS	0	0	12	NEG	NEG	0	0.086
11	6	25	POS	0	0	10	NEG	NEG	0	0.103
11	8	25	POS	1	1	9	NS	NS	NS	NS
11	12	25	POS	0	0	7	NEG	NEG	0	0.057
11	22	25	POS	0	0	4	NEG	POS	0	0.098
11	26	25	POS	0	0	4	NEG	POS	0	0.1
11	30	25	POS	0	0	0	NEG	NEG	0	0.106
11	34	25	POS	0	0	0	NEG	NEG	0	0.102
11	46	25	POS	0	0	0	NEG	NEG	0	0.057
11	50	25	POS	0	0	0	NEG	NEG	0	0.102
11	54	25	POS	0	0	0	NEG	NEG	0	0.041
11	58	25	POS	0	0	0	NEG	NEG	0	0
11	70	25	POS	0	0	0	NEG	NEG	0	0
11	74	25	POS	0	0	0	NEG	NEG	0	0
25	−1	25	NEG	0	0	0	NEG	NEG	0	0
25	0.17	25	NEG	0	0	0	POS	POS	110	0
25	0.5	25	NEG	0	0	0	POS	POS	57	0
25	1	25	POS	0	0	0	POS	POS	36	0
25	1.5	25	POS	0	0	0	NS	NS	18	0
25	2	25	POS	1	1	8	NS	NS	15	0
25	3	25	POS	3	2	16	NEG	NEG	3	0
25	4	25	POS	4	3	18	NEG	NEG	0	0.067
25	5	25	POS	2	2	15	NEG	NEG	0	0.033
25	6	25	POS	0	0	2	NEG	NEG	0	0.073
25	8	25	POS	0	0	5	NEG	POS	0	0.148
25	12	25	POS	0	0	0	NEG	NEG	0	0
25	22	25	POS	1	0	5	NEG	NEG	0	0
25	26	25	POS	0	0	8	NEG	NEG	0	0
25	30	25	POS	0	0	0	NEG	NEG	0	0
25	34	25	POS	0	0	0	NEG	NEG	0	0
27	−1	25	NEG	0	0	0	NEG	NEG	0	0
27	0.17	25	NEG	0	0	0	POS	POS	944	0
27	0.5	25	NEG	0	0	0	POS	POS	224	0
27	1	25	POS	0	0	0	POS	NEG	22	0
27	1.5	25	POS	3	3	12	POS	NEG	0	0
27	2	25	POS	3	2	12	POS	NEG	10	0
27	3	25	POS	0	2	10	NEG	NEG	0	0
27	4	25	POS	0	0	8	NEG	NEG	0	0.023
27	5	25	POS	0	0	7	NEG	NEG	0	0
27	6	25	POS	0	0	8	NEG	NEG	0	0
27	8	25	POS	0	0	5	NEG	NEG	0	0
27	12	25	POS	0	0	0	NEG	NEG	0	0
27	22	25	POS	0	0	0	NEG	NEG	0	0
27	26	25	POS	0	0	0	NEG	NEG	0	0
27	30	25	POS	0	0	0	NEG	NEG	0	0
27	34	25	POS	0	0	0	NEG	NEG	0	0
4	−1	50	NEG	0	0	0	NEG	NEG	0	0.062
4	0.17	50	NEG	0	0	0	POS	POS	479	0.122
4	0.5	50	NEG	0	0	0	POS	POS	492	NS
4	1	50	POS	1	2	12	NS	NS	NS	NS
4	1.5	50	POS	0	1	10	NS	NS	NS	0.067
4	2	50	POS	1	2	16	POS	POS	29	0
4	3	50	POS	1	2	11	POS	POS	168	0.052
4	4	50	POS	0	1	10	POS	NEG	24	0
4	5	50	POS	0	1	8	POS	NEG	3	0
4	6	50	POS	0	0	11	POS	NEG	2	0
4	8	50	NS	NS	NS	NS	POS	NEG	2	0
4	12	50	POS	0	0	0	NEG	NEG	0	0
4	22	50	POS	0	0	0	POS	NEG	0	0
7	−1	50	NEG	0	0	0	NEG	NEG	0	0
7	0.17	50	NEG	0	0	0	POS	POS	636	0
7	0.5	50	NEG	0	0	0	POS	POS	225	0
7	1	50	POS	4	1	9	POS	POS	162	0
7	1.5	50	POS	5	2	18	POS	POS	10	0.037
7	2	50	POS	5	3	27	POS	POS	3	0.08
7	3	50	POS	0	2	23	POS	POS	8	0.048
7	4	50	POS	2	1	17	NEG	NEG	0	0.048
7	5	50	POS	1	0	12	NEG	NEG	0	0.063
7	6	50	POS	0	0	11	NEG	NEG	0	0.062
7	8	50	POS	0	0	11	NEG	NEG	0	0.114
7	12	50	POS	2	0	17	NEG	NEG	0	0.096
7	22	50	POS	0	0	12	NEG	NEG	0	0.12
7	26	50	POS	0	0	9	NEG	POS	0	0.114
7	30	50	POS	0	0	8	NEG	POS	0	0.073
7	34	50	POS	0	0	7	NEG	NEG	0	0.087
7	46	50	POS	0	0	6	NEG	NEG	0	0.071
7	50	50	POS	0	0	4	NEG	POS	0	0.137
7	54	50	POS	0	0	5	NEG	POS	0	0.045
7	58	50	POS	0	0	4	NEG	NEG	0	0.096
7	70	50	POS	0	0	4	NEG	POS	0	0.028
7	74	50	POS	0	0	3	NEG	NEG	0	0.058
7	78	50	POS	0	0	3	NEG	NEG	0	0.042
7	82	50	POS	0	0	0	NEG	NEG	0	0.032
7	94	50	POS	0	0	3	NEG	NEG	0	0.021
7	98	50	POS	0	0	0	NEG	NEG	0	0.02
7	102	50	NEG	0	0	0	NEG	NEG	0	0
7	106	50	NEG	0	0	0	NEG	NEG	0	0.04
7	118	50	NEG	0	0	0	NEG	NEG	0	0.042
7	122	50	POS	0	0	0	NEG	NEG	0	0.04
7	126	50	NEG	0	0	0	NEG	NEG	0	0
17	−1	50	NEG	0	0	0	NEG	NEG	0	0
17	0.17	50	NEG	0	0	0	POS	POS	378	0
17	0.5	50	NEG	0	0	0	POS	POS	160	NS
17	1	50	POS	1	2	11	NS	NS	NS	NS
17	1.5	50	POS	1	3	22	NS	NS	NS	NS
17	2	50	POS	1	4	27	NS	NS	NS	NS
17	3	50	POS	1	3	34	POS	POS	NS	NS
17	4	50	POS	1	2	30	NS	NS	NS	0
17	5	50	POS	1	3	31	POS	POS	16	0
17	6	50	POS	0	2	26	POS	POS	6	0
17	8	50	POS	0	1	20	NEG	POS	0	0
17	12	50	POS	0	1	18	NEG	NEG	0	0
17	22	50	POS	0	0	12	NEG	NEG	0	0
17	26	50	POS	0	0	8	NEG	NEG	0	0
17	30	50	POS	0	0	14	NEG	NEG	0	0
17	34	50	POS	0	0	4	NEG	NEG	0	0
17	46	50	POS	0	0	3	NEG	NEG	0	0
17	50	50	POS	0	0	0	NEG	NEG	0	0
17	54	50	POS	0	0	0	NEG	NEG	0	0
17	58	50	POS	0	0	0	NEG	NEG	0	0
17	70	50	NEG	0	0	0	NEG	NEG	0	0
17	74	50	POS	0	0	0	NEG	NEG	0	0
19	−1	50	NEG	0	0	0	NEG	NEG	0	0.051
19	0.17	50	NEG	0	0	0	POS	POS	719	0.04
19	0.5	50	NEG	1	0	0	POS	POS	349	0.089
19	1	50	POS	4	3	10	NS	NS	75	0.129
19	1.5	50	POS	2	2	10	NS	NS	80	NS
19	2	50	POS	3	3	13	NEG	NEG	NS	0.451
19	3	50	POS	4	4	22	POS	POS	24	NS
19	4	50	POS	3	4	19	NS	NS	NS	0.367
19	5	50	POS	3	3	13	POS	POS	5	0.359
19	6	50	POS	3	3	14	POS	POS	2	0.269
19	8	50	POS	1	2	9	POS	POS	2	0.102
19	12	50	POS	0	0	8	NEG	NEG	0	0.642
19	22	50	POS	0	0	8	POS	POS	2	0.125
19	26	50	POS	0	0	6	NEG	POS	0	NS
19	30	50	POS	0	0	5	NEG	NEG	0	0.044
19	34	50	POS	0	0	4	NEG	NEG	0	0.264
19	46	50	POS	0	0	0	NEG	NEG	0	0
19	50	50	POS	0	0	0	NEG	NEG	0	0.041
19	54	50	POS	0	0	0	NEG	NEG	0	0
19	58	50	NEG	0	0	0	NEG	NEG	0	0.18
19	70	50	NEG	0	0	0	NEG	NEG	0	0.038
19	74	50	NEG	0	0	0	NEG	NEG	0	0
23	0	50	NEG	0	0	0	NEG	NEG	0	0.044
23	0.17	50	NEG	0	0	0	POS	POS	1010	0.063
23	0.5	50	POS	1	0	5	POS	POS	851	0.029
23	1	50	POS	2	1	16	POS	POS	196	0
23	1.5	50	POS	3	2	33	POS	NEG	4	0
23	2	50	POS	0	2	29	POS	POS	2	0.116
23	3	50	POS	5	2	32	POS	POS	11	0
23	4	50	POS	2	1	23	POS	POS	20	0
23	5	50	POS	1	1	26	POS	POS	24	0.02
23	6	50	POS	0	0	22	NEG	NEG	0	0.02
23	8	50	POS	0	0	14	NEG	NEG	0	0.099
23	12	50	POS	0	0	13	NEG	POS	2	0.163
23	22	50	POS	0	0	10	NEG	POS	0	0.052
23	26	50	POS	0	0	9	NEG	NEG	0	0
23	30	50	POS	0	0	5	NEG	NEG	0	0
23	34	50	POS	0	0	3	NEG	NEG	0	0
23	46	50	NEG	0	0	0	NEG	NEG	0	0
23	50	50	POS	0	0	0	NEG	NEG	0	0
24	−1	50	NEG	0	0	0	NEG	NEG	0	0
24	0.17	50	NEG	0	0	0	POS	POS	350	0
24	0.5	50	NEG	1	1	4	POS	POS	187	0
24	1	50	POS	3	3	17	POS	NEG	18	0
24	1.5	50	POS	2	4	27	POS	NEG	10	0.031
24	2	50	POS	2	4	30	POS	NEG	10	NS
24	3	50	POS	2	3	40	NS	NS	NS	0.089
24	4	50	NEG	3	3	37	NEG	NEG	2	0.337
24	5	50	POS	1	2	31	NS	NS	3	0.822
24	6	50	POS	4	2	37	NS	NS	7	0.095
24	8	50	POS	2	3	44	NEG	NEG	0	0.16
24	12	50	POS	0	1	17	NEG	NEG	0	0.067
24	22	50	POS	0	0	7	NEG	POS	0	0
24	26	50	POS	0	0	6	NEG	NEG	0	0.05
24	30	50	POS	0	0	4	NEG	NEG	0	0
24	34	50	POS	0	0	5	NEG	NEG	0	0.071
24	46	50	POS	0	0	3	NEG	NEG	0	0
24	50	50	POS	0	0	4	NEG	NEG	0	0
24	54	50	POS	0	0	4	NEG	NEG	0	0
24	58	50	POS	0	0	4	NEG	NEG	0	0
24	70	50	NEG	0	0	2	NEG	NEG	0	0
24	74	50	POS	0	0	3	NEG	NEG	0	0
24	78	50	POS	0	0	3	NEG	NEG	0	0
24	82	50	NEG	0	0	2	NEG	NEG	0	0
24	94	50	NEG	0	0	2	NEG	NEG	0	0

^aData are tabulated over time to the last specimen that a positive ELISA response was obtained or a measurable quantity of a THC analyte was recorded.

^bPOS = positive; NEG = negative; NS = no sample.