Figure 1. Protein purification.
Coomassie-stained denaturing gels of purified MBP-NBDs (A) and full-length SUR2A (B), SUR2B (C). Numbers adjacent to the gel indicate the molecular weights (mw, kDa). (A) Lanes: 1=NBD2A; 2=NBD2B; 3=NBD1; 4, mw markers. (B) Lanes: 1=SUR2A; 2, mw markers. (C) Lanes: 1, mw markers; Lane 2=SUR2B. Samples are purified eluates from affinity resins without further purification (A) or eluates from the gel filtration column (B, C).