Figure 3. ATPase activity of SUR2.
A, ATPase activity in the presence (●, n=4) or absence (○, n=1) of Mg2+ for purified SUR2A. B, ATPase activity of purified SUR2B with (●, n=3) or without (○, n=1) Mg2+. C. ATPase activity of SUR2A and SUR2B plotted on the same scale. The lines are fit to the Michaelis-Menton equation using Km=460μM, Vmax=2.52nmol Pi/min/mg and an offset of 0.1nmol Pi/min/mg for SUR2A, and Km=41μM, and Vmax=0.73nmol Pi/min/mg and an offset of 0.05 for SUR2B.