Skip to main content
. 2017 Sep 27;29(8):365–375. doi: 10.1093/intimm/dxx049

Fig. 4.

Fig. 4.

Low oxygen conditions induce TET enzyme expression and Foxp3 CNS2 demethylation in iTregs. (A) iTregs were induced in the presence of 10 µg ml−1 vitamin C (Vit.C) or under the 5% O2 condition. The expressions of indicated genes were analyzed by quantitative real-time PCR. (B) 5hmC content in iTregs induced with indicated conditions was determined by LC-MS/MS analysis. (C, D) Methylation status determined by bisulfite conversion of the CpG sites of Foxp3 CNS2 in iTregs generated under the 5% O2 condition and/or 10 µg ml−1 vitamin C (C), Ctla4, Ikzf4, Tnfrsf18 and Il2ra in iTregs generated under the 5% O2 condition and 10 µg ml−1 vitamin C (D). The methylation status of tTregs is shown as a positive control in (C). (E) Violin plot showing the genome-wide methylation levels of CpG sites under 5% oxygen and normoxic conditions as detected by SureSelect-PBAT. *P < 0.05.