Fig. 8.

Global DNA methylation and Dnmt1 expression in splenocytes increased after LPS stimulation in MRL/lpr mice based on diet source. (A) 5-mC DNA methylation ELISA. ELISA was performed on DNA extracted from splenocytes that were non-stimulated or stimulated for 24 h with LPS. (B) Dnmt1 mRNA expression. RNAs were extracted from splenocytes with and without LPS stimulation for 24 h. The Dnmt1 expression was quantified using the Taqman gene expression system. The graphs represent mean ± SEMs. *P < 0.05, ***P < 0.001, one-way ANOVA (A), t-test (B) (n = 4 mice per group).