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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cancer Res. 2017 Feb 27;15(7):884–895. doi: 10.1158/1541-7786.MCR-16-0444

Figure 3. Effect of VCAN knockdown on cell migration and invasion in ccRCC cells.

Figure 3

(A) After transfection of cells with siRNA’s for 48 hours, a wound was formed by scraping with a micropipette tip and closure measured after 16 (786-O) or 24 (Caki-2) hours by microscopy. Left: Representative images of wound healing assay. Right: Attenuation of VCAN significantly inhibited cell migration of both ccRCC cell lines. ***, P<0.001. (B) Invasive ability of cells was measured using Matrigel membrane. VCAN knockdown significantly decreased invasiveness of both ccRCC cell lines after 24 hours. Left: Representative images of invasion assay. Right: Invading cells were measured by absorbance at 560 nm. **, P<0.01. ***, P<0.001.