Skip to main content
. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cancer Res. 2017 Feb 27;15(7):884–895. doi: 10.1158/1541-7786.MCR-16-0444

Figure 4. Apoptosis-related genes altered by VCAN knockdown in ccRCC cells.

Figure 4

(A) Thirteen candidate apoptosis-related genes that were increased or decreased two-fold or greater after VCAN siRNA#2 treatment for 72 hours were identified by gene array analysis in 786-O cells. (B) Verification of gene array data by real-time PCR in Caki-2 and 786-O cells. Five upregulated genes (TNF-α, BIK, BAK, CD137, and TP53BP2) and 2 downregulated genes (IGF1R and BCL-2) were confirmed by real-time PCR using Taqman probes. *, P<0.05. **, P<0.01. ***, P<0.001. (C) Immunoblot analysis for apoptotic markers described in (B) and other TNF signaling pathway-related caspase genes in control and VCAN siRNA#2-transfected Caki-2 and 786-O cells. Shown are immunoblots with marked increase of decrease due to treatment. GAPDH used as a loading control.