SDS-PAGE (10%)/zymogram analysis of secreted and cell-associated forms of BglA. (A) SDS-PAGE stained with Coomassie brilliant blue R-250; (B) β-glucosidase zymogram gel. Lane S, protein molecular mass standards; lane 1, crude culture supernatant (10 μg); lane 2, purified secreted BglA (2 μg); lane 3, crude cell extract (60 μg); lane 4, partially purified BglA1 (2 μg); lane 5, partially purified BglA2 (2 μg); lane 6, purified secreted BglA (2 μg); lane 7, BglA1 (2 μg); lane 8, BglA2 (2 μg).