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Summary of Purification of Recombinant BglA from Culture Medium of S. cerevisiae
| Purification step | Total protein (mg) | Total units (μmol/min)a | Specific activity (μmol/[min·mg]) | Yield (%) |
|---|---|---|---|---|
| Culture filtrate | 480.00 | 326.8 | 0.68 | 100.0 |
| Concentrated supernatant | 165.30 | 319.7 | 1.93 | 97.8 |
| Phenyl Sepharose | 17.10 | 101.4 | 5.90 | 31.0 |
| Mono Q | 5.20 | 66.0 | 12.80 | 20.2 |
| Resource S | 2.23 | 38.3 | 17.20 | 11.7 |
| Superdex 200 | 0.17 | 3.2 | 18.80 | 1.0 |
Activities were measured with pNPG as substrate.
