Fig 4. Effect of ASB7 knockdown on protein levels of UPR markers under ER stress.

HeLa cells were transiently transfected pooling 2 independent ASB7 siRNAs, only ASB7 siRNA and compared with scrambled control. After 2 days, cells were cultured with Tun (20 μg/ml) for 6 h then total protein was extracted. Cell lysates were prepared, normalized for total protein and analyzed for immunoblotting for indicated proteins GRP78, CHOP, ATF4, IRE-1α, p-IRE-1α, p-eIF2α, eIF2α, p-p38 and p38 and normalized relative to the housekeeping gene β-Actin. Data representative of 2 experiments.