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. 2018 Apr 9;13(4):e0194310. doi: 10.1371/journal.pone.0194310

Fig 6. Effect of ASB7 knockdown on pro-inflammatory markers in ER stress.

Fig 6

(A) HeLa cells were transiently transfected pooling 2 independent ASB7 siRNA (gray bars) and compared with scrambled siRNA control (black bars) (SC). Cells were cultured with Tun (20 μg/ml) and DTT (2 mM) and total RNA was extracted. Levels of endogenous ASB4 mRNA levels were measured by qRT‐PCR for different time points 3, 6, 12 h and normalized relative to housekeeping gene, 18S rRNA (mean ± SEM, n = 2). “*” denotes significance with respect to scramble control. Error bars represent mean ± SEM. ***: p≤0.001; **: p≤0.01; *: p≤0.05 calculated using two-way ANOVA with Bonferroni correction. (B) HeLa cells were transiently transfected pooling 2 independent ASB7 siRNA (gray bars) and compared with scrambled siRNA control (black bars) (SC). After 2 days, total RNA was extracted for 6 h. Relative levels of mRNAs encoding TNF-α (left), IL-1β (center), and JUN (right) were compared by qRT-PCR and displayed as ratios relative to a housekeeping gene (18S rRNA). Data are mean ± SEM, n = 2. “*” denotes significance with respect to scramble control. Error bars represent mean ± SEM. *** p≤0.001; **: p≤0.01; *: p≤0.05 calculated using one-way ANOVA with Bonferroni correction.