Table 5. Nicotine contents of flower buds and seeds of tea plants (Yabukita), cultured tea cells and B5 medium before and after 9 days cultivation, and leaves, stems and roots of tea seedlings cultured under aseptic conditions for 90 days.

Samples		Nicotine content
		(μg g−1 dry weight)
Flower buds1)	Petals	0.031
	Filaments	0.041
	Anthers	0.033
Seeds1)		0.030
B5 medium2)	Before use	n.q.
	After cultivation	n.q.
Tea cells2)		0.033 ± 0.019
Tea seedlings1)	Leaves	0.033
	Stems	0.044
	Roots	0.059

¹⁾ Values are from a single replicate (n = 1).

²⁾ Values are means ± S.D. (n = 10). n.q.: not quantified [below the LOQ (0.005 μg g⁻¹ dry weight)].