Fig 5. A. thaliana seed permeability assay.

Seeds were incubated for 24 h in tetrazolium red at 28°C and formazan production assayed by measuring absorbance of the incubation solution at 492 nm. For plasma treated (purple bar), the air plasma was used and seeds were incubated with tetrazolium immediately following treatment. Control (blue bar) were treated similarly but without activating the plasma. Water (white bar) treatment omitted the tetrazolium. Bars show mean of 4 assays and the error bars are 0.05 (For data see S1 Fig).