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. 2018 Mar 28;16(3):e2002864. doi: 10.1371/journal.pbio.2002864

Fig 1. IF visualization of autophagy in anagen hair MKs from organ-cultured human HFs.

Fig 1

(I) Representative confocal image of organ-cultured anagen HFs probed with a specific anti-LC3B antibody/Alexa555 (red) and Hoechst 33342 nuclear staining (blue). (II and III) High magnification images showing the predominance of LC3B-positive dots in keratinocytes of the most proximal hair matrix and the precortical hair matrix. (IV) Three-dimensional reconstruction from high resolution confocal images showing the perinuclear distribution of LC3B-positive fluorescent dots, consistent with the recognized cellular localization of autophagosomes [23]. HF, hair follicle; IF, immunofluorescence; LC3B, Light Chain 3B; MK, matrix keratinocyte.