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Disruption of the TNF‐α/NF‐κB axis causes cell cycle arrest at the S phase. U2OS cells transfected with Con‐shRNA (Control) (A), U2OS‐SPD304 (B), U2OS‐TNFR1‐KD (C), U2OS‐RelA‐KD + RelA‐KD (D), U2OS‐TNFR1‐KD + RelB‐KD (E), U2OS‐TNFR1‐KD + c‐Rel‐KD (F), U2OS‐CUL4B‐KD (G), U2OS‐RelA‐KD + CUL4B‐KD (H), or U2OS‐p21‐OE (I) were subjected to flow cytometry analysis to determine cell cycle distribution. The cell cycle distributions from A to I were quantified in (J).
