Figure 7.

Effects of inhibition and activation of receptors on the phosphorylation of a panel of kinases in MKN7 cells. The Kinase Proteome Profiler was performed to detect the effects of trastuzumab and/or afatinib treatment on downstream kinases in MKN7 cells. The cells were treated for 20 min with 5 μg·mL⁻¹ trastuzumab, 0.5 μm afatinib, or the combination of both drugs or were stimulated for 5 min with 5 ng·mL⁻¹ EGF or 20 ng·mL⁻¹ NRG1. The phosphorylation levels were quantified using densitometric analysis, a linear mixture modeling was inferred, and cluster analysis was performed. In the cluster analysis, the x‐fold change of the activation of each included protein is shown. Samples were clustered due to the similarity of the activated proteins and treatment conditions. Significant effects between different treatment conditions are indicated by (*) with increasing size (0.01 < P‐value ≤ 0.05, 0.001 < P‐value ≤ 0.01, and P‐value ≤ 0.001). A, afatinib; T, trastuzumab; Ctrl, control.