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. 2018 Mar 15;12(4):561–576. doi: 10.1002/1878-0261.12185

Figure 5.

Figure 5

Efficacy of olaparib after initial androgen deprivation. Clonogenic assays were performed with LNCaP, LNCaP‐ABLR es, and DuCaP. In addition, LNCaP and DuCaP were grown for 3 weeks under +ABL conditions and then seeded for clonogenic assay under NGC (indicated as +ABL>NGC). (A) Plating efficiency (ratio of number of colonies per number of seeded cells) is depicted in absolute values. Statistically significant differences were calculated by Mann–Whitney test. (B, C) Clonogenic assays were performed with increasing concentrations of olaparib (0–2.5 μm), as indicated. Plating efficiencies relative to vehicle (DMSO) are indicated, and statistically significant differences were calculated by one‐way ANOVA (table) and Bonferroni post‐test (stars in graphs). (D, E) Tumor‐initiation experiments were performed with 1 × 104–105 LNCaP grown for 3 weeks under +ABL conditions followed by incubation in the RCCS with DMSO or 5 μm olaparib. (D) PSA was measured in the supernatant. Single values resulting from inoculations with different cell numbers are depicted, and statistically significant differences were calculated with Friedman test. (E) Tumor organoids were collected with a cell strainer. Scale bar, 2 mm. (A–D) NGC, normal growth conditions; +ABL, androgen deprivation mimicking ADT. *P < 0.05.