Fig 7. Sequencing of the molecular barcode isolated from mosquito #27 that fed on 776301.

Viral RNA that was isolated from the saliva, body, and legs of mosquito #27 at day 25 post feeding was converted into cDNA and then split into two separate PCR reactions with primer set A (178 bp) and B (131 bp). The theoretical number of cDNA molecules used in each PCR reaction is shown in Table 5. PCR products were tagged and sequenced. The frequency of each barcode detected was quantified. Mosquito #27 fed on 776301 on day 4, so the frequencies of barcodes detected in the plasma of 776301 from Fig 6 are shown. The frequency of any sequence in the region of the barcode that was not considered authentic is listed as ‘Other.’