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. 2018 Mar 28;14(3):e1006964. doi: 10.1371/journal.ppat.1006964

Table 2. Number of viral templates sequenced from nonpregnant animals infected with ZIKV-IC or ZIKV-BC-1.0.

Sample Number of vRNA templates put into the cDNA synthesis reaction Number of theoretical cDNA templates put into the PCR reaction*
296198 (ZIKV-IC) day 3 5255 626
296198 (ZIKV-IC) day 5 232,553 88,592
118693 (ZIKV-IC) day 3 1201 143
962498 (ZIKV-IC) day 3 3514 418
962498 (ZIKV-IC) day 5 4208 1,603
514982 (ZIKV-BC-1.0) day 2 146 14
715132 (ZIKV-BC-1.0) day 3 2865 341
715132 (ZIKV-BC-1.0) day 5 2821 1075
688387 (ZIKV-BC-1.0) day 3 667 79
688387 (ZIKV-BC-1.0) day 5 1526 581

*The number of cDNA templates was calculated based on the number of vRNA templates that were put into the cDNA synthesis reaction, and then the amount of cDNA that was used for the PCR reaction.