Table 2. Number of viral templates sequenced from nonpregnant animals infected with ZIKV-IC or ZIKV-BC-1.0.
Sample | Number of vRNA templates put into the cDNA synthesis reaction | Number of theoretical cDNA templates put into the PCR reaction* |
---|---|---|
296198 (ZIKV-IC) day 3 | 5255 | 626 |
296198 (ZIKV-IC) day 5 | 232,553 | 88,592 |
118693 (ZIKV-IC) day 3 | 1201 | 143 |
962498 (ZIKV-IC) day 3 | 3514 | 418 |
962498 (ZIKV-IC) day 5 | 4208 | 1,603 |
514982 (ZIKV-BC-1.0) day 2 | 146 | 14 |
715132 (ZIKV-BC-1.0) day 3 | 2865 | 341 |
715132 (ZIKV-BC-1.0) day 5 | 2821 | 1075 |
688387 (ZIKV-BC-1.0) day 3 | 667 | 79 |
688387 (ZIKV-BC-1.0) day 5 | 1526 | 581 |
*The number of cDNA templates was calculated based on the number of vRNA templates that were put into the cDNA synthesis reaction, and then the amount of cDNA that was used for the PCR reaction.