Table 5. Number of viral templates sequenced from one positive mosquito who fed on 776301.

Sample	Number of vRNA templates put into the cDNA synthesis reaction	Number of theoretical cDNA templates put into the PCR reaction*
Mosquito saliva	2830	1280
Mosquito body	10,000	4,524
Mosquito legs	10,000	4,524

*The number of cDNA templates was calculated based on the number of vRNA templates that were put into the cDNA synthesis reaction, and then the amount of cDNA that was used for the PCR reaction.