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. 2018 Mar 28;14(3):e1007277. doi: 10.1371/journal.pgen.1007277

Fig 8. Effect of 13xR mutation on ionizing radiation-induced foci formation by RAD52.

Fig 8

(A, B) MRC5V1-TR cells were unirradiated or irradiated with γ-rays (8 Gy), and subjected to immunofluorescent staining at the indicated time after irradiation. (A) Cells expressing FLAG-RAD52 (Wt)-HA were used. An anti-HA (green) antibody, an anti-γH2AX (red) antibody, and 4',6-diamidino-2-phenylindole (DAPI, blue) were used for immunofluorescent staining. (B) Cells expressing FLAG-RAD52 (13xR)-HA were used. An anti-HA (green) antibody, an anti-γ-tubulin (red) antibody, and DAPI (blue) were used for immunofluorescent staining. (C) MRC5V1-TR cells expressing FLAG-NLS-RAD52 (Wt)-HA or FLAG-NLS-RAD52 (13xR)-HA were irradiated with γ-rays (8 Gy), and subjected to immunofluorescent staining 6 h after irradiation. An anti-HA (green) antibody, an anti-γH2AX (red) antibody, and DAPI (blue) were used for immunofluorescent staining, and the overlaid images are shown.