Skip to main content
. Author manuscript; available in PMC: 2018 Jul 22.
Published in final edited form as: Nat Chem. 2018 Jan 22;10(3):318–324. doi: 10.1038/nchem.2927

Figure 5. Combinatorial codon mutagenesis sites and protocol.

Figure 5

a , Ribbon model of POP variant 0-ZA4 showing the location of residue 477 (green sphere), residue 413 (blue sphere), and residues selected for combinatorial codon mutagenesis (remaining spheres). Mutations in 1-RFY (Q98R, G99F, and P239Y) are shown as red, orange, and yellow spheres, respectively. b, Scaffold immobilization and ArM formation on Ni-NTA resin in 96-well plates using libraries of scaffold variants generated via error prone PCR or CCM as shown in Figure 2.