Figure 5.

Epithelial‐mesenchymal transition (EMT) ability of ABCG2+ pancreatic ductal adenocarcinoma cells derived from adherent culture condition. A, Immunocytochemical staining was performed in ABCG2− and ABCG2+ cells 48 h after culture with or without TGF‐β1 treatment. Representative images are shown (N‐cadherin, green). B, Quantitative RT‐PCR analysis of EMT markers was performed using cDNA derived from ABCG2− and ABCG2+ cells 48 h after adherent culture with or without TGF‐β1 treatment. The results are shown after normalization to the values obtained for ABCG2− cells without TGF‐β1 treatment (value = 1). Results are presented as means ± SD from 3 independent experiments