Figure 4.

Curcumin alters the expression of enzymes involved in testosterone metabolism. Protein expression in LNCaP (A) and 22Rv1 (B) were measured by western blot after 24‐h treatment with curcumin. The protein bands from top to bottom were Aldo‐Keto reductase 1C2 (AKR1C2), steroidogenic acute regulatory protein (StAR), Cytochrome P450scc (CYP11A1), type 2 3β‐hydroxysteroid dehydrogenase (HSD3B2), Cytochrome P450(17α) (CYP17A1), steroid 5α‐reductase type 1 (SRD5A1), steroid 5α‐reductase type 2 (SRD5A2) and β‐actin. Molecular weight is 37, 30, 60, 42, 55, 28, 29 and 42 KDa, respectively. Mouse anti‐human β‐actin antibody was used as an internal control