Figure 1.

NanoString assay for medulloblastoma subgrouping using the 33 gene CodeSet with the NanoString nCounter Analysis System (NanoString Technologies). Subgroups could be predicted in 96% (72/75) of tumors by non‐hierarchical clustering analysis, with 11.11% (8/72) of tumors subgrouped as WNT, 20.83% (15/72) as SHH, 25% (18/72) as Group 3, and 43.06% (31/72) as Group 4. Ten candidate subgroup signature genes were shown to have high subgroup specificity based on the classic gene panel assignment: PDGFRA,FGFR1, and ALK to the WNT group; CCND1,MYCN,TP53, and GLI1 to the SHH group; MYC to Group 3; and SNCAIP and SNCA to Group 4. Red indicates a high expression level of genes; green indicates low expression level