Figure 7.

Upregulation of EPHB4 by BC005927 mediates hypoxia‐induced gastric cancer (GC) metastasis. A1, A2, Protein levels of EPHB4 in SGC7901 and MKN45 cells. The cells were transfected with a BC005927 siRNA‐lentivirus or negative control (NC) and then exposed to hypoxia after transfection. The cells were then collected and subjected to western blot analysis at specific time points, as indicated. β‐Actin served as an internal control. B1, C1, Transwell migration and invasion assays of SGC7901‐NC and SGC7901‐si‐EPHB4 cells and MKN45‐NC and MKN28‐si‐EPHB4 cells were carried out under normoxic or hypoxic conditions. B2, C2, Protein levels of EPHB4 were determined by western blot assays. D1, D2, Transwell migration and invasion assays of SGC7901 cells were carried out after transfection with a BC005927 siRNA‐lentivirus, EPHB4 expression vector, or negative control (hypoxia) under normoxic or hypoxic conditions. Protein levels of EPHB4 were determined by western blot assays. In all panels, results are representative of at least 3 independent experiments