Figure 3.

SIM2 is involved in differentiation of esophageal squamous cell carcinoma (ESCC). A, Semiquantitative RT‐PCR of SIM2, FN1, VIM, SNAI2, TWIST1, PDPN, and ACTB in 2D cultured TE8 cells 3 days after transfection of pCMV6‐neo or pCMV‐AC‐GFP‐SIM2 (left) or pCMV6‐SIM2s (right). B, Photographic images of 3D cultured KYSE510, TE8, and T.Tn cells 3 days after transfection of pCMV6‐neo (upper) or pCMV‐AC‐GFP‐SIM2 (lower). C, Real‐time RT‐PCR of PDPN, VIM, SPRR1A, and FLG in 3D cultured KYSE510, TE8, and T.Tn cells 3 or 5 days after transfection of pCMV6‐neo (Control, white column) or pCMV‐AC‐GFP‐SIM2 (SIM2, red column) (n = 3, mean + SE; *P < .05)