Figure 1.

Reticulocalbin 1 (RCN1) is highly expressed in prostate tissues. A, Immunohistochemistry was used to determine RCN1, cyclin D1 and cyclin B. The statistical analysis of (B) RCN1, (C) cyclin D1 and (D) cyclin B were shown. *P < .05, **P < .01, n.s., no significance. E, Expression of RCN1 in prostate carcinoma was analyzed using the datasets from the Oncomine database. F, Survival analysis was shown using online microarray data with survival information between the high level of RCN1 mRNA with disease‐free survival in PCa patients (HR = 1.13, P = .067). G, The protein levels of RCN1 in RWPE1, PC3, DU145 and LNCaP were detected. H, Proliferation of PC3 cells transfected with pcDNA3.1‐RCN1 for 72 hours was detected using EdU. The charts indicate the number of EdU‐positive cells. I, PC3 and (J) DU145 were transfected with pcDNA3.1‐RCN1 for 72 hours. MTT assay and western blotting were performed. n.s., no significance