Skip to main content
. 2018 Mar 31;109(4):1147–1157. doi: 10.1111/cas.13541

Figure 2.

Figure 2

Reticulocalbin 1 (RCN1) depletion induces cell cycle arrest and cell death. A, DU145 and (B) LNCaP cells were transfected with siRCN1 for 48 or 72 hours. Cell cycle analyses were performed by flow cytometry, *P < .05. C, DU145 and (D) LNCaP cells were transfected with siRCN1 for 72 hours. Cell viability, morphological images and western blotting were obtained. **P < .01, ***P < .001. E, DU145 and (F) LNCaP cells were transfected with siRCN1 for 48 or 72 hours and stained with FITC‐conjugated annexin V and propidium iodide for flow cytometry. Statistical analysis is shown. *P < .05, **P < .01, ***P < .001, n.s., no significance. G, Changes of mitochondrial membrane potential (mtΔψ) were shown after RCN1 deletion in DU145 and LNCaP; *P < .05, ***P < .001