Figure 3.

Reticulocalbin 1 (RCN1) knockdown differentially induces caspase‐dependent apoptosis and necroptosis. A, Caspase‐3 activity was evaluated after transfecting 72 hours. **P < .01, n.s, no significance. B, Protein levels of RCN1 and PARP were performed by western blotting. C, DU145 and (D) LNCaP were pre‐treated with 20 μmol/L Z‐VAD‐FMK for 5 hours and transfected with negative control (NC) or siRCN1 for 48 hours. Cell viability was monitored using MTT assays. *P < .05, n.s., no significance. E, DU145 and (F) LNCaP cells were pre‐treated with 20 μmol/L Necrostatin‐1 for 5 hours and transfected with NC or siRCN1 for 48 hours. Cell viability was monitored using MTT assays. **P < .01, n.s., no significance