Figure 1.

Antimalarial drug mefloquine inhibited nuclear factor kappa B (NF‐κB)‐driven transactivation. A, HEK293T cells were transfected with luciferase reporter driven by NF‐κB response elements for 24 h, and treated with 104 drugs or DMSO for 12 h. Luciferase activity was determined using the Dual‐Luciferase Reporter Assay System. B, Chemical structure of mefloquine (Mef). C, HEK293T cells were transfected with vector or NFκB‐luc by Lipofectamine 2000 (Invitrogen) for 24 h. Cells were then treated with 0, 15, or 30 μmol/L mefloquine for 12 h. Luciferase activity was measured followed by stimulation with tumor necrosis factor alpha (TNF‐α) or control vehicle for 20 min. *P < .05, **P < .01