Figure 2.

Effect of KIAA0247 expression on the migration and invasion of non‐small‐cell lung cancer (NSCLC) cells. A549 and H1299 cells were transfected with KIAA0247‐specific siRNA (si‐KIAA0247) or control siRNA, or with a KIAA0247 expression plasmid (p‐KIAA0247) or vehicle (control). A, Cell migration analyzed by Transwell migration assay; cells that migrated to the lower chamber were stained with hematoxylin and counted. *P < .05; **P < .01. (i) A549 migration: si‐KIAA0247 vs control siRNA, P = .024; p‐KIAA0247 vs control, P = .005. (ii) H1299 migration: si‐KIAA0247 vs control siRNA, P = .046; p‐KIAA0247 vs control, P = .047. (iii) A549 invasion: si‐KIAA0247 vs control siRNA, P = .005; p‐KIAA0247 vs control P = .035. (iv) H1299 invasion: si‐KIAA0247 vs control siRNA, P = .007; p‐KIAA0247 vs control, P = .023. (B,C) Expression of KIAA0247 and cell migration‐ and invasion‐related proteins in transfected (B) A549 and (C) H1299 cells